Active site-directed proteomic probes for adenylation domains in nonribosomal peptide synthetases.

ABSTRACT: We describe a general strategy for selective chemical labeling of individual adenylation (A) domains in nonribosomal peptide synthetases (NRPSs) using active site-directed proteomic probes coupled to the 5'-O-N-(aminoacyl)sulfamoyladenosine (AMS) scaffold with a clickable benzophenone functionality. These proteomic tools can greatly facilitate the molecular identification, functional characterization, and profiling of virtually any kind of A domains of NRPS enzymes in complex biological systems.

SUBMITTER: Konno S 

PROVIDER: S-EPMC4795001 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature