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From one to many: expanding the Saccharomyces cerevisiae reference genome panel.

ABSTRACT: In recent years, thousands of Saccharomyces cerevisiae genomes have been sequenced to varying degrees of completion. The Saccharomyces Genome Database (SGD) has long been the keeper of the original eukaryotic reference genome sequence, which was derived primarily from S. cerevisiae strain S288C. Because new technologies are pushing S. cerevisiae annotation past the limits of any system based exclusively on a single reference sequence, SGD is actively working to expand the original S. cerevisiae systematic reference sequence from a single genome to a multi-genome reference panel. We first commissioned the sequencing of additional genomes and their automated analysis using the AGAPE pipeline. Here we describe our curation strategy to produce manually reviewed high-quality genome annotations in order to elevate 11 of these additional genomes to Reference status. Database URL: http://www.yeastgenome.org/.

SUBMITTER: Engel SR

PROVIDER: S-EPMC4795930 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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From one to many: expanding the Saccharomyces cerevisiae reference genome panel.

Engel Stacia R SR Weng Shuai S Binkley Gail G Paskov Kelley K Song Giltae G Cherry J Michael JM

Database : the journal of biological databases and curation 20160317

In recent years, thousands of Saccharomyces cerevisiae genomes have been sequenced to varying degrees of completion. The Saccharomyces Genome Database (SGD) has long been the keeper of the original eukaryotic reference genome sequence, which was derived primarily from S. cerevisiae strain S288C. Because new technologies are pushing S. cerevisiae annotation past the limits of any system based exclusively on a single reference sequence, SGD is actively working to expand the original S. cerevisiae ...[more]

PMID: 26989152

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