Ontology highlight
ABSTRACT:
SUBMITTER: Hazen JL
PROVIDER: S-EPMC4795965 | biostudies-literature | 2016 Mar
REPOSITORIES: biostudies-literature
Hazen Jennifer L JL Faust Gregory G GG Rodriguez Alberto R AR Ferguson William C WC Shumilina Svetlana S Clark Royden A RA Boland Michael J MJ Martin Greg G Chubukov Pavel P Tsunemoto Rachel K RK Torkamani Ali A Kupriyanov Sergey S Hall Ira M IM Baldwin Kristin K KK
Neuron 20160303 6
Somatic mutation in neurons is linked to neurologic disease and implicated in cell-type diversification. However, the origin, extent, and patterns of genomic mutation in neurons remain unknown. We established a nuclear transfer method to clonally amplify the genomes of neurons from adult mice for whole-genome sequencing. Comprehensive mutation detection and independent validation revealed that individual neurons harbor ∼100 unique mutations from all classes but lack recurrent rearrangements. Mos ...[more]