Ontology highlight
ABSTRACT:
SUBMITTER: Winkelman JT
PROVIDER: S-EPMC4797950 | biostudies-literature | 2016 Mar
REPOSITORIES: biostudies-literature
Winkelman Jared T JT Vvedenskaya Irina O IO Zhang Yuanchao Y Zhang Yu Y Bird Jeremy G JG Taylor Deanne M DM Gourse Richard L RL Ebright Richard H RH Nickels Bryce E BE
Science (New York, N.Y.) 20160301 6277
In bacterial transcription initiation, RNA polymerase (RNAP) selects a transcription start site (TSS) at variable distances downstream of core promoter elements. Using next-generation sequencing and unnatural amino acid-mediated protein-DNA cross-linking, we have determined, for a library of 4(10) promoter sequences, the TSS, the RNAP leading-edge position, and the RNAP trailing-edge position. We find that a promoter element upstream of the TSS, the "discriminator," participates in TSS selection ...[more]