Unknown

Dataset Information

0

Detection of intra-brain cytoplasmic 1 (BC1) long noncoding RNA using graphene oxide-fluorescence beacon detector.


ABSTRACT: Detection of cellular expression of long noncoding RNAs (lncRNAs) was elusive due to the ambiguity of exposure of their reactive sequences associated with their secondary/tertiary structures and dynamic binding of proteins around lncRNAs. Herein, we developed graphene-based detection techniques exploiting the quenching capability of graphene oxide (GO) flakes for fluorescent dye (FAM)-labeled single-stranded siRNAs and consequent un-quenching by their detachment from GO by matching lncRNAs. A brain cytoplasmic 1 (BC1) lncRNA expression was significantly decreased by a siRNA, siBC1-1. GO quenched the FAM-labeled siBC1-1 peptide nucleic acid (PNA) probe, and this quenching was recovered by BC1. While FAM-siBC1-1-PNA-GO complex transfected spontaneously mouse or human neural stem cells, fluorescence was recovered only in mouse cells having high BC1 expression. Fluorescent dye-labeled single-stranded RNA-GO probe could detect the reactive exposed nucleic acid sequence of a cytoplasmic lncRNA expressing in the cytoplasm, which strategy can be used as a detection method of lncRNA expression.

SUBMITTER: Kim MY 

PROVIDER: S-EPMC4800405 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature

altmetric image

Publications

Detection of intra-brain cytoplasmic 1 (BC1) long noncoding RNA using graphene oxide-fluorescence beacon detector.

Kim Mee Young MY   Hwang Do Won DW   Li Fangyuan F   Choi Yoori Y   Byun Jung Woo JW   Kim Dongho D   Kim Jee-Eun JE   Char Kookheon K   Lee Dong Soo DS  

Scientific reports 20160321


Detection of cellular expression of long noncoding RNAs (lncRNAs) was elusive due to the ambiguity of exposure of their reactive sequences associated with their secondary/tertiary structures and dynamic binding of proteins around lncRNAs. Herein, we developed graphene-based detection techniques exploiting the quenching capability of graphene oxide (GO) flakes for fluorescent dye (FAM)-labeled single-stranded siRNAs and consequent un-quenching by their detachment from GO by matching lncRNAs. A br  ...[more]

Similar Datasets

| S-EPMC7644748 | biostudies-literature
| S-EPMC3216571 | biostudies-literature
| S-EPMC3494035 | biostudies-literature
| S-EPMC2577337 | biostudies-literature
| S-EPMC6071027 | biostudies-literature
| S-EPMC6800036 | biostudies-literature
| S-EPMC8044317 | biostudies-literature
| S-EPMC7912907 | biostudies-literature
| S-EPMC5855184 | biostudies-literature
| S-EPMC7064105 | biostudies-literature