Characterization of an in vitro fed-batch model to obtain cells released from S. epidermidis biofilms.

ABSTRACT: Both dynamic and fed-batch systems have been used for the study of biofilms. Dynamic systems, whose hallmark is the presence of continuous flow, have been considered the most appropriate for the study of the last stage of the biofilm lifecycle: biofilm disassembly. However, fed-batch is still the most used system in the biofilm research field. Hence, we have used a fed-batch system to collect cells released from Staphylococcus epidermidis biofilms, one of the most important etiological agents of medical device-associated biofilm infections. Herein, we showed that using this model it was possible to collect cells released from biofilms formed by 12 different S. epidermidis clinical and commensal isolates. In addition, our data indicated that biofilm disassembly occurred by both passive and

SUBMITTER: Franca A 

PROVIDER: S-EPMC4801823 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature