Ontology highlight
ABSTRACT:
SUBMITTER: Kamiyama D
PROVIDER: S-EPMC4802074 | biostudies-literature | 2016 Mar
REPOSITORIES: biostudies-literature
Kamiyama Daichi D Sekine Sayaka S Barsi-Rhyne Benjamin B Hu Jeffrey J Chen Baohui B Gilbert Luke A LA Ishikawa Hiroaki H Leonetti Manuel D MD Marshall Wallace F WF Weissman Jonathan S JS Huang Bo B
Nature communications 20160318
In addition to the popular method of fluorescent protein fusion, live cell protein imaging has now seen more and more application of epitope tags. The small size of these tags may reduce functional perturbation and enable signal amplification. To address their background issue, we adapt self-complementing split fluorescent proteins as epitope tags for live cell protein labelling. The two tags, GFP11 and sfCherry11 are derived from the eleventh β-strand of super-folder GFP and sfCherry, respectiv ...[more]