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Identification of ?-phenylalanine as a non-protein amino acid in cultivated rice, Oryza sativa.


ABSTRACT: Non-protein amino acids, often analogs of the standard 20 protein amino acids, have been discovered in many plant species. Recent research with cultivated rice (Oryza sativa) identified (3R)-?-tyrosine, as well as a tyrosine amino mutase that synthesizes (3R)-?-tyrosine from the protein amino acid (2S)-?-tyrosine. Gas chromatography-mass spectrometry (GC-MS) assays and comparison to an authentic standard showed that ?-phenylalanine is also a relatively abundant non-protein amino acid in rice leaves and that its biosynthesis occurs independently from that of ?-tyrosine.

SUBMITTER: Yokoo T 

PROVIDER: S-EPMC4802750 | biostudies-literature | 2015 Sep-Oct

REPOSITORIES: biostudies-literature

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Identification of β-phenylalanine as a non-protein amino acid in cultivated rice, Oryza sativa.

Yokoo Takayuki T   Takata Ryo R   Yan Jian J   Matsumoto Fuka F   Teraishi Masayoshi M   Okumoto Yutaka Y   Jander Georg G   Mori Naoki N  

Communicative & integrative biology 20150925 5


Non-protein amino acids, often analogs of the standard 20 protein amino acids, have been discovered in many plant species. Recent research with cultivated rice (Oryza sativa) identified (3R)-β-tyrosine, as well as a tyrosine amino mutase that synthesizes (3R)-β-tyrosine from the protein amino acid (2S)-α-tyrosine. Gas chromatography-mass spectrometry (GC-MS) assays and comparison to an authentic standard showed that β-phenylalanine is also a relatively abundant non-protein amino acid in rice lea  ...[more]

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