Ontology highlight
ABSTRACT:
SUBMITTER: Tie HC
PROVIDER: S-EPMC4803310 | biostudies-literature | 2016 Mar
REPOSITORIES: biostudies-literature
Tie Hieng Chiong HC Mahajan Divyanshu D Chen Bing B Cheng Li L VanDongen Antonius M J AM Lu Lei L
Molecular biology of the cell 20160113 5
Cellular functions of the Golgi are determined by the unique distribution of its resident proteins. Currently, electron microscopy is required for the localization of a Golgi protein at the sub-Golgi level. We developed a quantitative sub-Golgi localization method based on centers of fluorescence masses of nocodazole-induced Golgi ministacks under conventional optical microscopy. Our method is rapid, convenient, and quantitative, and it yields a practical localization resolution of ∼ 30 nm. The ...[more]