Ontology highlight
ABSTRACT:
SUBMITTER: Jennings MJ
PROVIDER: S-EPMC4803570 | biostudies-literature | 2016 May
REPOSITORIES: biostudies-literature
Jennings Matthew J MJ Barrios Adam F AF Tan Song S
Protein expression and purification 20151229
Undesirable truncated recombinant protein products pose a special expression and purification challenge because such products often share similar chromatographic properties as the desired full length protein. We describe here our observation of both full length and a truncated form of a yeast protein (Gcn5) expressed in Escherichia coli, and the reduction or elimination of the truncated form by mutating a cryptic Shine-Dalgarno or START codon within the Gcn5 coding region. Unsuccessful attempts ...[more]