Multiple Lytic Origins of Replication Are Required for Optimal Gammaherpesvirus Fitness In Vitro and In Vivo.
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ABSTRACT:
SUBMITTER: Sattler C
PROVIDER: S-EPMC4805163 | biostudies-literature | 2016 Mar
REPOSITORIES: biostudies-literature
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Multiple Lytic Origins of Replication Are Required for Optimal Gammaherpesvirus Fitness In Vitro and In Vivo.
Sattler Christine C Steer Beatrix B Adler Heiko H
PLoS pathogens 20160323 3
An unresolved question in herpesvirus biology is why some herpesviruses contain more than one lytic origin of replication (oriLyt). Using murine gammaherpesvirus 68 (MHV-68) as model virus containing two oriLyts, we demonstrate that loss of either of the two oriLyts was well tolerated in some situations but not in others both in vitro and in vivo. This was related to the cell type, the organ or the route of inoculation. Depending on the cell type, different cellular proteins, for example Hexim1 ...[more]
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