Dataset Information

Net-Immobilization of ?-glucosidase on Nonwoven Fabrics to Lower the Cost of "Cellulosic Ethanol" and Increase Cellulose Conversions.

ABSTRACT: The main limitation preventing the use of enzymatic cellulosic ethanol in industrial production is its higher cost which is mainly due to the elevated price of ?-glucosidase (BG). Herein, we report on a simple strategy for the in-situ encapsulation of BG for repeated cellulosic ethanol production. In this strategy, BG was net-immobilized into a poly(ethylene glycol) (PEG) net-cloth layer on a PP nonwoven fabric by way of the visible light-induced surface controlled/living graft cross-linking polymerization. The visible light and mild reaction conditions could ensure the activity retention of BG during immobilization, while the non-swelling uniform net-mesh formed by living cross-linking polymerization could prevent the leakage of BG effectively (at the immobilization rate of more than 98.6% and the leakage rate of only 0.4%). When the BG-loaded fabric was used in combination with free cellulase (CEL), the results of the catalytic reaction demonstrated that these BG-loaded fabrics could not only give a 40% increase in cellulose conversions but also be reused for more than fifteen batches without losing the activity. These BG-loaded fabrics with characteristics including easy separation, excellent operation stability, a low cost of the polymeric matrix and a simple fabrication process are particularly interesting for a future bio-fuel production strategy.

SUBMITTER: Zhu X

PROVIDER: S-EPMC4806303 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature

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Publications

Net-Immobilization of β-glucosidase on Nonwoven Fabrics to Lower the Cost of "Cellulosic Ethanol" and Increase Cellulose Conversions.

Zhu Xing X He Bin B Zhao Changwen C Fan Rong R Zhang Lihua L Wang Guan G Ma Yuhong Y Yang Wantai W

Scientific reports 20160324

The main limitation preventing the use of enzymatic cellulosic ethanol in industrial production is its higher cost which is mainly due to the elevated price of β-glucosidase (BG). Herein, we report on a simple strategy for the in-situ encapsulation of BG for repeated cellulosic ethanol production. In this strategy, BG was net-immobilized into a poly(ethylene glycol) (PEG) net-cloth layer on a PP nonwoven fabric by way of the visible light-induced surface controlled/living graft cross-linking pol ...[more]

PMID: 27009788

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Dataset Information

Net-Immobilization of ?-glucosidase on Nonwoven Fabrics to Lower the Cost of "Cellulosic Ethanol" and Increase Cellulose Conversions.

Publications

Net-Immobilization of β-glucosidase on Nonwoven Fabrics to Lower the Cost of "Cellulosic Ethanol" and Increase Cellulose Conversions.

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