Modular synthesis of N-glycans and arrays for the hetero-ligand binding analysis of HIV antibodies.

ABSTRACT: A new class of broadly neutralizing antibodies (bNAbs) from HIV donors has been reported to target the glycans on gp120--a glycoprotein found on the surface of the virus envelope--thus renewing hope of developing carbohydrate-based HIV vaccines. However, the version of gp120 used in previous studies was not from human T cells and so the glycosylation pattern could be somewhat different to that found in the native system. Moreover, some antibodies recognized two different glycans simultaneously and this cannot be detected with the commonly used glycan microarrays on glass slides. Here, we have developed a glycan microarray on an aluminium-oxide-coated glass slide containing a diverse set of glycans, including homo- and mixed N-glycans (high-mannose, hybrid and complex types) that were prepa

SUBMITTER: Shivatare SS 

PROVIDER: S-EPMC4806563 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature