Anti-Inflammatory Activity of Triterpenes Isolated from Protium paniculatum Oil-Resins.

ABSTRACT: Protium is the main genus of the Burseraceae family and one of the most common genera in South America, with an important species called "breu." Gum and oil-resins of this species are used as tonic and stimulant and for the treatment of ulcers and inflammation. The present study aims to isolate and investigate the anti-inflammatory activity of triterpene compounds isolated from oil-resin of Protium paniculatum. The pentacyclic triterpenes ?,?-amyrin, acetylated ?,?-amyrin, ?,?-amyrone, and brein/maniladiol did not alter the viability of murine J774 macrophages (IC50 > 20?µg/mL), with the exception of mixture of brein/maniladiol which showed moderate cytotoxic activity. Also it was observed that compounds at 10?µg/mL inhibited more than 80% of production of NO(•), although only ?,?-amyrin was able to inhibit the production of TNF-? (52.03 ± 2.4%). The compounds inhibited the production of IL-6 and induced the production of IL-10 in murine J774 macrophages stimulated by LPS. ?,?-Amyrone inhibited the expression of COX-2 and also inhibited the formation of paw or ear edema in rats and mice, having a quick and immediate effect. This study may provide the basis for future investigations on the therapeutic role of ?,?-amyrone in treating inflammation.

SUBMITTER: de Almeida PD 

PROVIDER: S-EPMC4806667 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature