Ontology highlight
ABSTRACT:
SUBMITTER: Murray CI
PROVIDER: S-EPMC4807126 | biostudies-literature | 2016 Jan
REPOSITORIES: biostudies-literature
Murray Christopher I CI Westhoff Maartje M Eldstrom Jodene J Thompson Emely E Emes Robert R Fedida David D
eLife 20160123
Cardiac repolarization is determined in part by the slow delayed rectifier current (IKs), through the tetrameric voltage-gated ion channel, KCNQ1, and its β-subunit, KCNE1. The stoichiometry between α and β-subunits has been controversial with studies reporting either a strict 2 KCNE1:4 KCNQ1 or a variable ratio up to 4:4. We used IKs fusion proteins linking KCNE1 to one (EQ), two (EQQ) or four (EQQQQ) KCNQ1 subunits, to reproduce compulsory 4:4, 2:4 or 1:4 stoichiometries. Whole cell and single ...[more]