Mitigation of NADPH Oxidase 2 Activity as a Strategy to Inhibit Peroxynitrite Formation.
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ABSTRACT:
SUBMITTER: Zielonka J
PROVIDER: S-EPMC4807286 | biostudies-literature | 2016 Mar
REPOSITORIES: biostudies-literature
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Mitigation of NADPH Oxidase 2 Activity as a Strategy to Inhibit Peroxynitrite Formation.
Zielonka Jacek J Zielonka Monika M VerPlank Lynn L Cheng Gang G Hardy Micael M Ouari Olivier O Ayhan Mehmet Menaf MM Podsiadły Radosław R Sikora Adam A Lambeth J David JD Kalyanaraman Balaraman B
The Journal of biological chemistry 20160202 13
Using high throughput screening-compatible assays for superoxide and hydrogen peroxide, we identified potential inhibitors of the NADPH oxidase (Nox2) isoform from a small library of bioactive compounds. By using multiple probes (hydroethidine, hydropropidine, Amplex Red, and coumarin boronate) with well defined redox chemistry that form highly diagnostic marker products upon reaction with superoxide (O2 (̇̄)), hydrogen peroxide (H2O2), and peroxynitrite (ONOO(-)), the number of false positives ...[more]
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