Solution Structure of CCL19 and Identification of Overlapping CCR7 and PSGL-1 Binding Sites.
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ABSTRACT:
SUBMITTER: Veldkamp CT
PROVIDER: S-EPMC4809050 | biostudies-literature | 2015 Jul
REPOSITORIES: biostudies-literature
Publications
Solution Structure of CCL19 and Identification of Overlapping CCR7 and PSGL-1 Binding Sites.
Veldkamp Christopher T CT Kiermaier Eva E Gabel-Eissens Skylar J SJ Gillitzer Miranda L ML Lippner David R DR DiSilvio Frank A FA Mueller Casey J CJ Wantuch Paeton L PL Chaffee Gary R GR Famiglietti Michael W MW Zgoba Danielle M DM Bailey Asha A AA Bah Yaya Y Engebretson Samantha J SJ Graupner David R DR Lackner Emily R ER LaRosa Vincent D VD Medeiros Tysha T Olson Michael L ML Phillips Andrew J AJ Pyles Harley H Richard Amanda M AM Schoeller Scott J SJ Touzeau Boris B Williams Larry G LG Sixt Michael M Peterson Francis C FC
Biochemistry 20150706 27
CCL19 and CCL21 are chemokines involved in the trafficking of immune cells, particularly within the lymphatic system, through activation of CCR7. Concurrent expression of PSGL-1 and CCR7 in naive T-cells enhances recruitment of these cells to secondary lymphoid organs by CCL19 and CCL21. Here the solution structure of CCL19 is reported. It contains a canonical chemokine domain. Chemical shift mapping shows the N-termini of PSGL-1 and CCR7 have overlapping binding sites for CCL19 and binding is c ...[more]
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