Characterization of Enzymatic Activity of MlrB and MlrC Proteins Involved in Bacterial Degradation of Cyanotoxins Microcystins.
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ABSTRACT:
SUBMITTER: Dziga D
PROVIDER: S-EPMC4810221 | biostudies-literature | 2016 Mar
REPOSITORIES: biostudies-literature
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Characterization of Enzymatic Activity of MlrB and MlrC Proteins Involved in Bacterial Degradation of Cyanotoxins Microcystins.
Dziga Dariusz D Zielinska Gabriela G Wladyka Benedykt B Bochenska Oliwia O Maksylewicz Anna A Strzalka Wojciech W Meriluoto Jussi J
Toxins 20160316 3
Bacterial degradation of toxic microcystins produced by cyanobacteria is a common phenomenon. However, our understanding of the mechanisms of these processes is rudimentary. In this paper several novel discoveries regarding the action of the enzymes of the mlr cluster responsible for microcystin biodegradation are presented using recombinant proteins. In particular, the predicted active sites of the recombinant MlrB and MlrC were analyzed using functional enzymes and their inactive muteins. A ne ...[more]
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