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Ca2+/H+ exchange by acidic organelles regulates cell migration in vivo.

ABSTRACT: Increasing evidence implicates Ca(2+) in the control of cell migration. However, the underlying mechanisms are incompletely understood. Acidic Ca(2+) stores are fast emerging as signaling centers. But how Ca(2+) is taken up by these organelles in metazoans and the physiological relevance for migration is unclear. Here, we identify a vertebrate Ca(2+)/H(+)exchanger (CAX) as part of a widespread family of homologues in animals. CAX is expressed in neural crest cells and required for their migration in vivo. It localizes to acidic organelles, tempers evoked Ca(2+) signals, and regulates cell-matrix adhesion during migration. Our data provide new molecular insight into how Ca(2+) is handled by acidic organelles and link this to migration, thereby underscoring the role of noncanonical Ca(2+) stores in the control of Ca(2+)-dependent function.

SUBMITTER: Melchionda M

PROVIDER: S-EPMC4810305 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature

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Ca2+/H+ exchange by acidic organelles regulates cell migration in vivo.

Melchionda Manuela M Pittman Jon K JK Mayor Roberto R Patel Sandip S

The Journal of cell biology 20160321 7

Increasing evidence implicates Ca(2+) in the control of cell migration. However, the underlying mechanisms are incompletely understood. Acidic Ca(2+) stores are fast emerging as signaling centers. But how Ca(2+) is taken up by these organelles in metazoans and the physiological relevance for migration is unclear. Here, we identify a vertebrate Ca(2+)/H(+)exchanger (CAX) as part of a widespread family of homologues in animals. CAX is expressed in neural crest cells and required for their migratio ...[more]

PMID: 27002171

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