Ontology highlight
ABSTRACT:
SUBMITTER: Mukherjee S
PROVIDER: S-EPMC4811770 | biostudies-literature | 2016 Mar
REPOSITORIES: biostudies-literature
Mukherjee Shatanik S Jansen Vera V Jikeli Jan F JF Hamzeh Hussein H Alvarez Luis L Dombrowski Marco M Balbach Melanie M Strünker Timo T Seifert Reinhard R Kaupp U Benjamin UB Wachten Dagmar D
eLife 20160322
The cellular messenger cAMP regulates multiple cellular functions, including signaling in cilia and flagella. The cAMP dynamics in these subcellular compartments are ill-defined. We introduce a novel FRET-based cAMP biosensor with nanomolar sensitivity that is out of reach for other sensors. To measure cAMP dynamics in the sperm flagellum, we generated transgenic mice and reveal that the hitherto methods determining total cAMP levels do not reflect changes in free cAMP levels. Moreover, cAMP dyn ...[more]