Ontology highlight
ABSTRACT:
SUBMITTER: Feaga HA
PROVIDER: S-EPMC4814080 | biostudies-literature | 2016 Mar
REPOSITORIES: biostudies-literature
Feaga Heather A HA Quickel Michael D MD Hankey-Giblin Pamela A PA Keiler Kenneth C KC
PLoS genetics 20160330 3
Bacteria use trans-translation and the alternative rescue factors ArfA (P36675) and ArfB (Q9A8Y3) to hydrolyze peptidyl-tRNA on ribosomes that stall near the 3' end of an mRNA during protein synthesis. The eukaryotic protein ICT1 (Q14197) is homologous to ArfB. In vitro ribosome rescue assays of human ICT1 and Caulobacter crescentus ArfB showed that these proteins have the same activity and substrate specificity. Both ArfB and ICT1 hydrolyze peptidyl-tRNA on nonstop ribosomes or ribosomes stalle ...[more]