Ontology highlight
ABSTRACT:
SUBMITTER: Kirscht A
PROVIDER: S-EPMC4814140 | biostudies-literature | 2016 Mar
REPOSITORIES: biostudies-literature
Kirscht Andreas A Kaptan Shreyas S SS Bienert Gerd Patrick GP Chaumont François F Nissen Poul P de Groot Bert L BL Kjellbom Per P Gourdon Pontus P Johanson Urban U
PLoS biology 20160330 3
Aquaporins of the TIP subfamily (Tonoplast Intrinsic Proteins) have been suggested to facilitate permeation of water and ammonia across the vacuolar membrane of plants, allowing the vacuole to efficiently sequester ammonium ions and counteract cytosolic fluctuations of ammonia. Here, we report the structure determined at 1.18 Å resolution from twinned crystals of Arabidopsis thaliana aquaporin AtTIP2;1 and confirm water and ammonia permeability of the purified protein reconstituted in proteolipo ...[more]