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Imaging multicellular specimens with real-time optimized tiling light-sheet selective plane illumination microscopy.

ABSTRACT: Despite the progress made in selective plane illumination microscopy, high-resolution 3D live imaging of multicellular specimens remains challenging. Tiling light-sheet selective plane illumination microscopy (TLS-SPIM) with real-time light-sheet optimization was developed to respond to the challenge. It improves the 3D imaging ability of SPIM in resolving complex structures and optimizes SPIM live imaging performance by using a real-time adjustable tiling light sheet and creating a flexible compromise between spatial and temporal resolution. We demonstrate the 3D live imaging ability of TLS-SPIM by imaging cellular and subcellular behaviours in live C. elegans and zebrafish embryos, and show how TLS-SPIM can facilitate cell biology research in multicellular specimens by studying left-right symmetry breaking behaviour of C. elegans embryos.

SUBMITTER: Fu Q

PROVIDER: S-EPMC4814582 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature

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Imaging multicellular specimens with real-time optimized tiling light-sheet selective plane illumination microscopy.

Fu Qinyi Q Martin Benjamin L BL Matus David Q DQ Gao Liang L

Nature communications 20160323

Despite the progress made in selective plane illumination microscopy, high-resolution 3D live imaging of multicellular specimens remains challenging. Tiling light-sheet selective plane illumination microscopy (TLS-SPIM) with real-time light-sheet optimization was developed to respond to the challenge. It improves the 3D imaging ability of SPIM in resolving complex structures and optimizes SPIM live imaging performance by using a real-time adjustable tiling light sheet and creating a flexible com ...[more]

PMID: 27004937

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