Unknown

Dataset Information

0

Untargeted Metabolomics Reveals Dose-Response Characteristics for Effect of Rhubarb in a Rat Model of Cholestasis.


ABSTRACT: Cholestasis is a serious manifestation of liver diseases with limited therapies. Rhubarb, a widely used herbal medicine, has been frequently used at a relatively large dose for treating cholestasis. However, whether large doses are optimal and the therapeutic mechanism remain unclear. To explore these questions, the anti-cholestatic effect of five doses of rhubarb (0.21, 0.66, 2.10, 6.60, and 21.0 g/kg) in an alpha-naphthylisothiocyanate (ANIT)-induced rat model of cholestasis was examined by histopathology and serum biochemistry. A dose-dependent anti-cholestatic effect of rhubarb (0.21-6.6 g/kg) was observed, and an overdose of 21.0 g/kg showed a poor effect. LC-MS-based untargeted metabolomics together with pathway analysis were further applied to characterize the metabolic alterations induced by the different rhubarb doses. Altogether, 13 biomarkers were identified. The dose-response curve based on nine important biomarkers indicated that doses in the 0.42-6.61 g/kg range (EC20-EC80 range, corresponding to 4.00-62.95 g in the clinic) were effective for cholestasis treatment. The pathway analysis showed that bile acid metabolism and excretion, inflammation and amino acid metabolism were altered by rhubarb in a dose-dependent manner and might be involved in the dose-response relationship and therapeutic mechanism of rhubarb for cholestasis treatment.

SUBMITTER: Zhang CE 

PROVIDER: S-EPMC4814850 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

altmetric image

Publications

Untargeted Metabolomics Reveals Dose-Response Characteristics for Effect of Rhubarb in a Rat Model of Cholestasis.

Zhang Cong-En CE   Niu Ming M   Li Rui-Yu RY   Feng Wu-Wen WW   Ma Xiao X   Dong Qin Q   Ma Zhi-Jie ZJ   Li Guang-Quan GQ   Meng Ya-Kun YK   Wang Ya Y   Yin Ping P   He Lan-Zhi LZ   Li Yu-Meng YM   Tan Peng P   Zhao Yan-Ling YL   Wang Jia-Bo JB   Dong Xiao-Ping XP   Xiao Xiao-He XH  

Frontiers in pharmacology 20160331


Cholestasis is a serious manifestation of liver diseases with limited therapies. Rhubarb, a widely used herbal medicine, has been frequently used at a relatively large dose for treating cholestasis. However, whether large doses are optimal and the therapeutic mechanism remain unclear. To explore these questions, the anti-cholestatic effect of five doses of rhubarb (0.21, 0.66, 2.10, 6.60, and 21.0 g/kg) in an alpha-naphthylisothiocyanate (ANIT)-induced rat model of cholestasis was examined by hi  ...[more]

Similar Datasets

| S-EPMC9144515 | biostudies-literature
| S-EPMC7313215 | biostudies-literature
| S-EPMC4904104 | biostudies-literature
| S-EPMC10538058 | biostudies-literature
| S-EPMC4585987 | biostudies-literature
| S-EPMC5925442 | biostudies-literature
| S-EPMC4334135 | biostudies-literature
| S-EPMC7594276 | biostudies-literature
| S-EPMC6331458 | biostudies-literature
| S-EPMC7569447 | biostudies-literature