Ontology highlight
ABSTRACT:
SUBMITTER: Miller MT
PROVIDER: S-EPMC4815302 | biostudies-literature | 2016 Jan
REPOSITORIES: biostudies-literature
Protein science : a publication of the Protein Society 20150918 1
The development of a modified DNA aptamer that binds HIV-1 reverse transcriptase (RT) with ultra-high affinity has enabled the X-ray structure determination of an HIV-1 RT-DNA complex to 2.3 Å resolution without the need for an antibody Fab fragment or RT-DNA cross-linking. The 38-mer hairpin-DNA aptamer has a 15 base-pair duplex, a three-deoxythymidine hairpin loop, and a five-nucleotide 5'-overhang. The aptamer binds RT in a template-primer configuration with the 3'-end positioned at the polym ...[more]