Unknown

Dataset Information

0

IRE1-RACK1 axis orchestrates ER stress preconditioning-elicited cytoprotection from ischemia/reperfusion injury in liver.


ABSTRACT: Endoplasmic reticulum (ER) stress is involved in ischemic preconditioning that protects various organs from ischemia/reperfusion (I/R) injury. We established an in vivo ER stress preconditioning model in which tunicamycin was injected into rats before hepatic I/R. The hepatic I/R injury, demonstrated by serum aminotransferase level and the ultra-structure of the liver, was alleviated by administration of tunicamycin, which induced ER stress in rat liver by activating inositol-requiring enzyme 1 (IRE1) and upregulating 78 kDa glucose-regulated protein (GRP78). The proteomic identification for IRE1 binders revealed interaction and cooperation among receptor for activated C kinase 1 (RACK1), phosphorylated AMPK, and IRE1 under ER stress conditions in a spatiotemporal manner. Furthermore, in vitro ER stress preconditioning was induced by thapsigargin and tunicamycin in L02 and HepG2 cells. Surprisingly, BCL2 was found to be phosphorylated by IRE1 under ER stress conditions to prevent apoptotic process by activation of autophagy. In conclusion, ER stress preconditioning protects against hepatic I/R injury, which is orchestrated by IRE1-RACK1 axis through the activation of BCL2. Our findings provide novel insights into the molecular pathways underlying ER stress preconditioning-elicited cytoprotective effect against hepatic I/R injury.

SUBMITTER: Liu D 

PROVIDER: S-EPMC4816147 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature

altmetric image

Publications

IRE1-RACK1 axis orchestrates ER stress preconditioning-elicited cytoprotection from ischemia/reperfusion injury in liver.

Liu Dong D   Liu Xing X   Zhou Ti T   Yao William W   Zhao Jun J   Zheng Zhigang Z   Jiang Wei W   Wang Fengsong F   Aikhionbare Felix O FO   Hill Donald L DL   Emmett Nerimah N   Guo Zhen Z   Wang Dongmei D   Yao Xuebiao X   Chen Yong Y  

Journal of molecular cell biology 20151228 2


Endoplasmic reticulum (ER) stress is involved in ischemic preconditioning that protects various organs from ischemia/reperfusion (I/R) injury. We established an in vivo ER stress preconditioning model in which tunicamycin was injected into rats before hepatic I/R. The hepatic I/R injury, demonstrated by serum aminotransferase level and the ultra-structure of the liver, was alleviated by administration of tunicamycin, which induced ER stress in rat liver by activating inositol-requiring enzyme 1  ...[more]

Similar Datasets

| S-EPMC4186414 | biostudies-literature
| S-EPMC4588407 | biostudies-literature
| S-EPMC5955491 | biostudies-literature
| S-EPMC8313843 | biostudies-literature
| S-EPMC5218813 | biostudies-literature
| S-EPMC7191926 | biostudies-literature
| S-EPMC6336234 | biostudies-literature
| S-EPMC4770158 | biostudies-literature
| S-EPMC3730367 | biostudies-literature
| S-EPMC4637556 | biostudies-literature