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Cas9 Functionally Opens Chromatin.

ABSTRACT: Using a nuclease-dead Cas9 mutant, we show that Cas9 reproducibly induces chromatin accessibility at previously inaccessible genomic loci. Cas9 chromatin opening is sufficient to enable adjacent binding and transcriptional activation by the settler transcription factor retinoic acid receptor at previously unbound motifs. Thus, we demonstrate a new use for Cas9 in increasing surrounding chromatin accessibility to alter local transcription factor binding.

SUBMITTER: Barkal AA

PROVIDER: S-EPMC4816323 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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Cas9 Functionally Opens Chromatin.

Barkal Amira A AA Srinivasan Sharanya S Hashimoto Tatsunori T Gifford David K DK Sherwood Richard I RI

PloS one 20160331 3

Using a nuclease-dead Cas9 mutant, we show that Cas9 reproducibly induces chromatin accessibility at previously inaccessible genomic loci. Cas9 chromatin opening is sufficient to enable adjacent binding and transcriptional activation by the settler transcription factor retinoic acid receptor at previously unbound motifs. Thus, we demonstrate a new use for Cas9 in increasing surrounding chromatin accessibility to alter local transcription factor binding. ...[more]

PMID: 27031353

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