Ontology highlight
ABSTRACT:
SUBMITTER: Walker OS
PROVIDER: S-EPMC4821487 | biostudies-literature | 2016 Jan
REPOSITORIES: biostudies-literature
Walker Olivia S OS Elsässer Simon J SJ Mahesh Mohan M Bachman Martin M Balasubramanian Shankar S Chin Jason W JW
Journal of the American Chemical Society 20160112 3
Isocitrate dehydrogenase is mutated at a key active site arginine residue (Arg172 in IDH2) in many cancers, leading to the synthesis of the oncometabolite (R)-2-hydroxyglutarate (2HG). To investigate the early events following acquisition of this mutation in mammalian cells we created a photoactivatable version of IDH2(R172K), in which K172 is replaced with a photocaged lysine (PCK), via genetic code expansion. Illumination of cells expressing this mutant protein led to a rapid increase in the l ...[more]