Direct-methods structure determination of a trypanosome RNA-editing substrate fragment with translational pseudosymmetry.
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ABSTRACT:
SUBMITTER: Mooers BH
PROVIDER: S-EPMC4822560 | biostudies-literature | 2016 Apr
REPOSITORIES: biostudies-literature
Publications
Direct-methods structure determination of a trypanosome RNA-editing substrate fragment with translational pseudosymmetry.
Acta crystallographica. Section D, Structural biology 20160324 Pt 4
Using direct methods starting from random phases, the crystal structure of a 32-base-pair RNA (675 non-H RNA atoms in the asymmetric unit) was determined using only the native diffraction data (resolution limit 1.05 Å) and the computer program SIR2014. The almost three helical turns of the RNA in the asymmetric unit introduced partial or imperfect translational pseudosymmetry (TPS) that modulated the intensities when averaged by the l Miller indices but still escaped automated detection. Almost ...[more]
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