Potential use of bacterial community succession for estimating post-mortem interval as revealed by high-throughput sequencing.

ABSTRACT: Decomposition is a complex process involving the interaction of both biotic and abiotic factors. Microbes play a critical role in the process of carrion decomposition. In this study, we analysed bacterial communities from live rats and rat remains decomposed under natural conditions, or excluding sarcosaphagous insect interference, in China using Illumina MiSeq sequencing of 16S rRNA gene amplicons. A total of 1,394,842 high-quality sequences and 1,938 singleton operational taxonomic units were obtained. Bacterial communities showed notable variation in relative abundance and became more similar to each other across body sites during the decomposition process. As decomposition progressed, Proteobacteria (mostly Gammaproteobacteria) became the predominant phylum in both the buccal cavity an

SUBMITTER: Guo J 

PROVIDER: S-EPMC4823735 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature