Unknown

Dataset Information

0

Phosphorylation and dephosphorylation regulate APC/C(Cdh1) substrate degradation.


ABSTRACT: The Anaphase Promoting Complex/Cyclosome (APC/C) ubiquitin ligase activated by its G1 specific adaptor protein Cdh1 is a major regulator of the cell cycle. The APC/C(Cdh1) mediates degradation of dozens of proteins, however, the kinetics and requirements for their degradation are largely unknown. We demonstrate that overexpression of the constitutive active CDH1(m11) mutant that is not inhibited by phosphorylation results in mitotic exit in the absence of the FEAR and MEN pathways, and DNA re-replication in the absence of Cdc7 activity. This mode of mitotic exit also reveals additional requirements for APC/C(Cdh1) substrate degradation, which for some substrates such as Pds1 or Clb5 is dephosphorylation, but for others such as Cdc5 is phosphorylation.

SUBMITTER: Simpson-Lavy KJ 

PROVIDER: S-EPMC4825543 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

altmetric image

Publications

Phosphorylation and dephosphorylation regulate APC/C(Cdh1) substrate degradation.

Simpson-Lavy Kobi J KJ   Zenvirth Drora D   Brandeis Michael M  

Cell cycle (Georgetown, Tex.) 20150101 19


The Anaphase Promoting Complex/Cyclosome (APC/C) ubiquitin ligase activated by its G1 specific adaptor protein Cdh1 is a major regulator of the cell cycle. The APC/C(Cdh1) mediates degradation of dozens of proteins, however, the kinetics and requirements for their degradation are largely unknown. We demonstrate that overexpression of the constitutive active CDH1(m11) mutant that is not inhibited by phosphorylation results in mitotic exit in the absence of the FEAR and MEN pathways, and DNA re-re  ...[more]

Similar Datasets

| S-EPMC3703968 | biostudies-literature
| S-EPMC6338662 | biostudies-literature
| S-EPMC2262036 | biostudies-literature
| S-EPMC3158779 | biostudies-literature
| S-EPMC3738489 | biostudies-literature
| S-EPMC4401652 | biostudies-literature
| S-EPMC4300845 | biostudies-literature
| S-EPMC4945139 | biostudies-other
| S-EPMC4195823 | biostudies-literature
| S-EPMC3243670 | biostudies-literature