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The Coding Region of the HCV Genome Contains a Network of Regulatory RNA Structures.


ABSTRACT: RNA is a versatile macromolecule that accommodates functional information in primary sequence and secondary and tertiary structure. We use a combination of chemical probing, RNA structure modeling, comparative sequence analysis, and functional assays to examine the role of RNA structure in the hepatitis C virus (HCV) genome. We describe a set of conserved but functionally diverse structural RNA motifs that occur in multiple coding regions of the HCV genome, and we demonstrate that conformational changes in these motifs influence specific stages in the virus' life cycle. Our study shows that these types of structures can pervade a genome, where they play specific mechanistic and regulatory roles, constituting a "code within the code" for controlling biological processes.

SUBMITTER: Pirakitikulr N 

PROVIDER: S-EPMC4826301 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature

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The Coding Region of the HCV Genome Contains a Network of Regulatory RNA Structures.

Pirakitikulr Nathan N   Kohlway Andrew A   Lindenbach Brett D BD   Pyle Anna M AM  

Molecular cell 20160225 1


RNA is a versatile macromolecule that accommodates functional information in primary sequence and secondary and tertiary structure. We use a combination of chemical probing, RNA structure modeling, comparative sequence analysis, and functional assays to examine the role of RNA structure in the hepatitis C virus (HCV) genome. We describe a set of conserved but functionally diverse structural RNA motifs that occur in multiple coding regions of the HCV genome, and we demonstrate that conformational  ...[more]

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