Dataset Information

Investigating the effect of drought stress on expression of WRKY1 and EREBP1 genes and antioxidant enzyme activities in lemon balm (Melissa Officinalis L.).

ABSTRACT: Drought stress is a severe environmental constraint to plant productivity. In this study, gene expression of WRKY1 and EREBP1 genes and activity of two enzymes were investigated in two genotypes, G9 (as resistance) and G12 (as susceptible) of 10 days-old lemon balm seedlings. Experiments were done according to factorial design on the base of completely randomized design with three replications for expression analysis and enzyme assays. Seedlings were cultured in MS medium suspensions, including 0, 3, 6, 12 and 15 % w/v PEG 6000. Leaf samples were subsequently collected at 0, 3, 6, 24, 48, 72 h after culture. According to the results of the enzyme assay, SOD activity in resistant genotype was more than in sensitive one. POD activity was high in G9 in severe drought condition and in G12 this activity was increased at initial times of drought stress. The survey results revealed that the expression levels of both genes, EREBP1 and WRKY1, in G9 variety were more than G12, and in this respect we can say that the G9 variety is more resistant to drought.

SUBMITTER: Rahimi M

PROVIDER: S-EPMC4826382 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature

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Investigating the effect of drought stress on expression of WRKY1 and EREBP1 genes and antioxidant enzyme activities in lemon balm (Melissa Officinalis L.).

Rahimi Mehdi M Kordrostami Mojtaba M Maleki Mahmood M ModaresKia Mohsen M

3 Biotech 20160408 1

Drought stress is a severe environmental constraint to plant productivity. In this study, gene expression of WRKY1 and EREBP1 genes and activity of two enzymes were investigated in two genotypes, G9 (as resistance) and G12 (as susceptible) of 10 days-old lemon balm seedlings. Experiments were done according to factorial design on the base of completely randomized design with three replications for expression analysis and enzyme assays. Seedlings were cultured in MS medium suspensions, including ...[more]

PMID: 28330169

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Project description:BackgroundNowadays, light-emitting diodes (LEDs) as a new lighting technology, have been emerged as an alternative source of light for plants due to their wavelength specificity, the narrow width of their bands, small size, solid structure, long lifetime, and low heat generation. Here we investigated the effect of different LED light sources on the essential oil components and antioxidant activity of Melissa officinalis. Two genotypes of lemon balm (Ilam and Isfahan) were subjected to four artificial light treatments, including white, red, blue, red + blue LEDs, and greenhouse light as natural lighting.ResultsThe LED lights significantly increased shoot fresh and dry weights and leaf number in the two genotypes as compared to greenhouse condition. The results showed that the content and composition of essential oil in the two genotypes were variable under different light treatments and the total amount of compounds in the Ilam genotype was higher than the other genotype. The results of analysis of the essential oil by GC/MS indicated that the highest amount of monoterpenes in the genotypes was related to citronellal under red + blue LED lamps (15.3 and 17.2% in Ilam and Isfahan genotypes, respectively) but blue, white, and greenhouse condition had the most effect on sesquiterpenes content in both genotypes. The results showed that the observed variation between the two genotypes in the essentials oil composition was related to the relative percentage of the constituents and not to the appearance or lack of a specific component. Red + blue lighting also provided the highest radical scavenging activity in both genotypes (80.77 and 82.09% for Ilam and Isfahan genotypes, respectively). Based on principal component analyses (PCA), three main groups were identified regarding genotypes and all light treatments.ConclusionsOverall, results indicated that the essentials oil composition of two genotypes of lemon balm was affected both qualitatively and quantitatively by different LED light sources; hence, LED lights might be used to improve monoterpenes, sesquiterpenes, and antioxidant activity in the selected genotypes.

Dataset Information

Investigating the effect of drought stress on expression of WRKY1 and EREBP1 genes and antioxidant enzyme activities in lemon balm (Melissa Officinalis L.).

Publications

Investigating the effect of drought stress on expression of WRKY1 and EREBP1 genes and antioxidant enzyme activities in lemon balm (Melissa Officinalis L.).

Similar Datasets

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