Single cell analysis in native tissue: Quantification of the retinoid content of hepatic stellate cells.
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ABSTRACT:
SUBMITTER: Galler K
PROVIDER: S-EPMC4827054 | biostudies-literature | 2016 Apr
REPOSITORIES: biostudies-literature
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Single cell analysis in native tissue: Quantification of the retinoid content of hepatic stellate cells.
Galler Kerstin K Requardt Robert Pascal RP Glaser Uwe U Markwart Robby R Bocklitz Thomas T Bauer Michael M Popp Jürgen J Neugebauer Ute U
Scientific reports 20160411
Hepatic stellate cells (HSCs) are retinoid storing cells in the liver: The retinoid content of those cells changes depending on nutrition and stress level. There are also differences with regard to a HSC's anatomical position in the liver. Up to now, retinoid levels were only accessible from bulk measurements of tissue homogenates or cell extracts. Unfortunately, they do not account for the intercellular variability. Herein, Raman spectroscopy relying on excitation by the minimally destructive w ...[more]
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