Ontology highlight
ABSTRACT:
SUBMITTER: Min J
PROVIDER: S-EPMC4827060 | biostudies-literature | 2016 Apr
REPOSITORIES: biostudies-literature
Min Junseon J Song Eun Kyung EK Kim Hansol H Kim Kyoung Taek KT Park Tae Joo TJ Kang Sebyung S
Scientific reports 20160411
We construct a novel recombinant secondary antibody mimic, GST-ABD, which can bind to the Fc regions of target-bound primary antibodies and acquire multiple HRPs simultaneously. We produce it in tenth of mg quantities with a bacterial overexpression system and simple purification procedures, significantly reducing the manufacturing cost and time without the use of animals. GST-ABD is effectively conjugated with 3 HRPs per molecule on an average and selectively bind to the Fc region of primary an ...[more]