Ontology highlight
ABSTRACT:
SUBMITTER: Coren LV
PROVIDER: S-EPMC4827251 | biostudies-literature | 2015 Mar
REPOSITORIES: biostudies-literature
Coren Lori V LV Jain Sumiti S Trivett Matthew T MT Ohlen Claes C Ott David E DE
BioTechniques 20150301 3
Here we present an improved strategy for producing T-cell receptor (TCR)-expressing retroviral vectors using a Golden Gate cloning strategy. This method takes advantage of the modular nature of TCR genes by directly amplifying TCR α and β variable regions from RNA or cDNA, then cloning and fusing them with their respective constant region genes resident in a retroviral TCR expression vector. Our one-step approach greatly streamlines the TCR vector production process in comparison to the traditio ...[more]