Ontology highlight
ABSTRACT:
SUBMITTER: Kumar M
PROVIDER: S-EPMC4829111 | biostudies-literature | 2016 Apr
REPOSITORIES: biostudies-literature
Kumar Manoj M Kovalski Letícia L Broyles David D Hunt Eric A EA Daftarian Pirouz P Dikici Emre E Daunert Sylvia S Deo Sapna K SK
Analytical biochemistry 20160107
Here we describe the design and construction of an imaging construct with high bioluminescent resonance energy transfer (BRET) efficiency that is composed of multiple quantum dots (QDs; λem = 655 nm) self-assembled onto a bioluminescent protein, Renilla luciferase (Rluc). This is facilitated by the streptavidin-biotin interaction, allowing the facile formation of a hybrid-imaging construct (HIC) comprising up to six QDs (acceptor) grafted onto a light-emitting Rluc (donor) core. The resulting as ...[more]