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A minimalist biosensor: Quantitation of cyclic di-GMP using the conformational change of a riboswitch aptamer.

ABSTRACT: Cyclic di-GMP (c-di-GMP) is a second messenger that is important in regulating bacterial physiology and behavior, including motility and virulence. Many questions remain about the role and regulation of this signaling molecule, but current methods of detection are limited by either modest sensitivity or requirements for extensive sample purification. We have taken advantage of a natural, high affinity receptor of c-di-GMP, the Vc2 riboswitch aptamer, to develop a sensitive and rapid electrophoretic mobility shift assay (EMSA) for c-di-GMP quantitation that required minimal engineering of the RNA.

SUBMITTER: Kellenberger CA

PROVIDER: S-EPMC4829349 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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A minimalist biosensor: Quantitation of cyclic di-GMP using the conformational change of a riboswitch aptamer.

Kellenberger Colleen A CA Sales-Lee Jade J Pan Yuchen Y Gassaway Madalee M MM Herr Amy E AE Hammond Ming C MC

RNA biology 20150626 11

Cyclic di-GMP (c-di-GMP) is a second messenger that is important in regulating bacterial physiology and behavior, including motility and virulence. Many questions remain about the role and regulation of this signaling molecule, but current methods of detection are limited by either modest sensitivity or requirements for extensive sample purification. We have taken advantage of a natural, high affinity receptor of c-di-GMP, the Vc2 riboswitch aptamer, to develop a sensitive and rapid electrophore ...[more]

PMID: 26114964

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