Ontology highlight
ABSTRACT:
SUBMITTER: Huang Q
PROVIDER: S-EPMC4829451 | biostudies-literature | 2016 Jun
REPOSITORIES: biostudies-literature
Huang Qiongying Q Gershenson Anne A Roberts Mary F MF
Biochimica et biophysica acta 20160311 6
The broad-range phospholipase C (PLC) from Listeria monocytogenes has been expressed using an intein expression system and characterized. This zinc metalloenzyme, similar to the homologous enzyme from Bacillus cereus, targets a wide range of lipid substrates. With monomeric substrates, the length of the hydrophobic acyl chain has significant impact on enzyme efficiency by affecting substrate affinity (Km). Based on a homology model of the enzyme to the B. cereus protein, several active site resi ...[more]