Ontology highlight
ABSTRACT:
SUBMITTER: Mandegar MA
PROVIDER: S-EPMC4830697 | biostudies-literature | 2016 Apr
REPOSITORIES: biostudies-literature
Mandegar Mohammad A MA Huebsch Nathaniel N Frolov Ekaterina B EB Shin Edward E Truong Annie A Olvera Michael P MP Chan Amanda H AH Miyaoka Yuichiro Y Holmes Kristin K Spencer C Ian CI Judge Luke M LM Gordon David E DE Eskildsen Tilde V TV Villalta Jacqueline E JE Horlbeck Max A MA Gilbert Luke A LA Krogan Nevan J NJ Sheikh Søren P SP Weissman Jonathan S JS Qi Lei S LS So Po-Lin PL Conklin Bruce R BR
Cell stem cell 20160310 4
Developing technologies for efficient and scalable disruption of gene expression will provide powerful tools for studying gene function, developmental pathways, and disease mechanisms. Here, we develop clustered regularly interspaced short palindromic repeat interference (CRISPRi) to repress gene expression in human induced pluripotent stem cells (iPSCs). CRISPRi, in which a doxycycline-inducible deactivated Cas9 is fused to a KRAB repression domain, can specifically and reversibly inhibit gene ...[more]