An improved smaller biotin ligase for BioID proximity labeling.
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ABSTRACT:
SUBMITTER: Kim DI
PROVIDER: S-EPMC4831873 | biostudies-literature | 2016 Apr
REPOSITORIES: biostudies-literature
Publications
An improved smaller biotin ligase for BioID proximity labeling.
Kim Dae In DI Jensen Samuel C SC Noble Kyle A KA Kc Birendra B Roux Kenneth H KH Motamedchaboki Khatereh K Roux Kyle J KJ
Molecular biology of the cell 20160224 8
The BioID method uses a promiscuous biotin ligase to detect protein-protein associations as well as proximate proteins in living cells. Here we report improvements to the BioID method centered on BioID2, a substantially smaller promiscuous biotin ligase. BioID2 enables more-selective targeting of fusion proteins, requires less biotin supplementation, and exhibits enhanced labeling of proximate proteins. Thus BioID2 improves the efficiency of screening for protein-protein associations. We also de ...[more]
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