Unknown

Dataset Information

0

Autophagy is dispensable for B-cell development but essential for humoral autoimmune responses.

ABSTRACT: To gain new insight into the role of B-cell autophagy, we generated two novel mouse models deficient for the autophagy-related gene (Atg)5, one from the outset pro-B cell stage (Atg5(f/-) Mb1 cre) and the other in mature B cells only (Atg5(f/-) CD21 cre). We show that autophagy is dispensable for pro- to pre-B cell transition, but necessary at a basal level to maintain normal numbers of peripheral B cells. It appears non-essential for B-cell activation under B-cell receptor stimulation but required for their survival after lipopolysaccharide stimulation that drives plasmablast differentiation and for specific IgM production after immunization. Results obtained using Atg5(f/-) CD21 cre × C57BL/6(lpr/lpr) autoimmune-prone mice show that B-cell autophagy is involved in the maintenance of anti-nuclear antibody secretion, elevated number of long-lived plasma cells, and sustains IgG deposits in the kidneys. Thus, treatments specifically targeting autophagy might be beneficial in systemic autoimmune diseases.

SUBMITTER: Arnold J 

PROVIDER: S-EPMC4832104 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Autophagy is dispensable for B-cell development but essential for humoral autoimmune responses.

Arnold J J   Murera D D   Arbogast F F   Fauny J-D JD   Muller S S   Gros F F  

Cell death and differentiation 20151120 5

To gain new insight into the role of B-cell autophagy, we generated two novel mouse models deficient for the autophagy-related gene (Atg)5, one from the outset pro-B cell stage (Atg5(f/-) Mb1 cre) and the other in mature B cells only (Atg5(f/-) CD21 cre). We show that autophagy is dispensable for pro- to pre-B cell transition, but necessary at a basal level to maintain normal numbers of peripheral B cells. It appears non-essential for B-cell activation under B-cell receptor stimulation but requi  ...[more]

Similar Datasets

Unknown Bi-Allelic TCR? or ? Recombination Enhances T Cell Development but Is Dispensable for Antigen Responses and Experimental Autoimmune Encephalomyelitis.
| S-EPMC4687847 | biostudies-literature
Unknown Essential role for autophagy during invariant NKT cell development.
| S-EPMC4284579 | biostudies-literature
Unknown Humoral Responses to Diverse Autoimmune Disease-Associated Antigens in Multiple Sclerosis.
| S-EPMC4466031 | biostudies-literature
Unknown Foxp4 is dispensable for T cell development, but required for robust recall responses.
| S-EPMC3418275 | biostudies-literature
Unknown T<sub>FR</sub> Cells Express Functional CCR6 But It Is Dispensable for Their Development and Localization During Splenic Humoral Immune Responses.
| S-EPMC9257258 | biostudies-literature
Unknown Ets1 Controls the Development of B Cell Autoimmune Responses in a Cell-Intrinsic Manner.
| S-EPMC7008956 | biostudies-literature
Unknown SUMO2 is essential while SUMO3 is dispensable for mouse embryonic development.
| S-EPMC4197045 | biostudies-literature
Transcriptomics Autophagy is essential for cardiac morphogenesis during vertebrate development
2014-03-01 | E-GEOD-51541 | biostudies-arrayexpress
Unknown Celastrus-derived celastrol suppresses autoimmune arthritis by modulating antigen-induced cellular and humoral effector responses.
| S-EPMC3083183 | biostudies-literature
Unknown The pluripotency-associated gene Dppa4 is dispensable for embryonic stem cell identity and germ cell development but essential for embryogenesis.
| S-EPMC2682008 | biostudies-literature