Ontology highlight
ABSTRACT:
SUBMITTER: Rho HS
PROVIDER: S-EPMC4833427 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
Rho Hoon Suk HS Hanke Alexander Thomas AT Ottens Marcel M Gardeniers Han H
PloS one 20160415 4
A microfluidic platform or "microfluidic mapper" is demonstrated, which in a single experiment performs 36 parallel biochemical reactions with 36 different combinations of two reagents in stepwise concentration gradients. The volume used in each individual reaction was 36 nl. With the microfluidic mapper, we obtained a 3D enzyme reaction plot of horseradish peroxidase (HRP) with Amplex Red (AR) and hydrogen peroxide (H2O2), for concentration ranges of 11.7 μM to 100.0 μM and 11.1 μM to 66.7 μM f ...[more]