Wnt/?-Catenin Stimulation and Laminins Support Cardiovascular Cell Progenitor Expansion from Human Fetal Cardiac Mesenchymal Stromal Cells.
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ABSTRACT:
SUBMITTER: Mansson-Broberg A
PROVIDER: S-EPMC4834052 | biostudies-literature | 2016 Apr
REPOSITORIES: biostudies-literature
Publications
Wnt/β-Catenin Stimulation and Laminins Support Cardiovascular Cell Progenitor Expansion from Human Fetal Cardiac Mesenchymal Stromal Cells.
Månsson-Broberg Agneta A Rodin Sergey S Bulatovic Ivana I Ibarra Cristián C Löfling Marie M Genead Rami R Wärdell Eva E Felldin Ulrika U Granath Carl C Alici Evren E Le Blanc Katarina K Smith C I Edvard CIE Salašová Alena A Westgren Magnus M Sundström Erik E Uhlén Per P Arenas Ernest E Sylvén Christer C Tryggvason Karl K Corbascio Matthias M Simonson Oscar E OE Österholm Cecilia C Grinnemo Karl-Henrik KH Grinnemo Karl-Henrik KH
Stem cell reports 20160324 4
The intrinsic regenerative capacity of human fetal cardiac mesenchymal stromal cells (MSCs) has not been fully characterized. Here we demonstrate that we can expand cells with characteristics of cardiovascular progenitor cells from the MSC population of human fetal hearts. Cells cultured on cardiac muscle laminin (LN)-based substrata in combination with stimulation of the canonical Wnt/β-catenin pathway showed increased gene expression of ISL1, OCT4, KDR, and NKX2.5. The majority of cells staine ...[more]
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