Room temperature electrocompetent bacterial cells improve DNA transformation and recombineering efficiency.
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ABSTRACT:
SUBMITTER: Tu Q
PROVIDER: S-EPMC4837392 | biostudies-literature | 2016 Apr
REPOSITORIES: biostudies-literature
Publications
Room temperature electrocompetent bacterial cells improve DNA transformation and recombineering efficiency.
Tu Qiang Q Yin Jia J Fu Jun J Herrmann Jennifer J Li Yuezhong Y Yin Yulong Y Stewart A Francis AF Müller Rolf R Zhang Youming Y
Scientific reports 20160420
Bacterial competent cells are essential for cloning, construction of DNA libraries, and mutagenesis in every molecular biology laboratory. Among various transformation methods, electroporation is found to own the best transformation efficiency. Previous electroporation methods are based on washing and electroporating the bacterial cells in ice-cold condition that make them fragile and prone to death. Here we present simple temperature shift based methods that improve DNA transformation and recom ...[more]
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