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Functional characterization and microencapsulation of probiotic bacteria from koozh.

ABSTRACT: Koozh is a traditional fermented millet beverage unique to south India. Analysis of six market samples of koozh for their microbial profile resulted in 69 isolates of presumptive lactic acid bacteria (LAB). They were grouped as Leuconostoc sp., Enterococcus sp., Streptococcus sp. and Lactobacillus sp. based on morphological characteristics and biochemical tests. Eight among them showed probiotic features: resistance to acid (2.5 pH for 6 h), resistance to 0.3 % ox bile, moderate hydrophobicity (40 %), antibacterial activity against 10 pathogens, susceptibility to 50 % of antibiotics tested. Sequencing of 16srDNA showed them to be five strains of Enterococcus hirae and one each of Enterococcus facecalis, Bacillus amyloliquefaciens and Lactobacillus plantarum. The probiotic isolates were encapsulated in skim milk powder by two different drying techniques: freeze drying and spray drying. The encapsulated probiotic isolates survived both in simulated gastric fluid and simulated intestinal fluid with high cell viability (98-99 %). Storage for 16 weeks at room temperature (27 °C), resulted in 2 log reduction, but better survival with only 1 log reduction was observed at 4 °C and was best at -20 °C. Survival of isolates was similar in both spray and freeze dried products.

SUBMITTER: Ilango S

PROVIDER: S-EPMC4837729 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature

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Functional characterization and microencapsulation of probiotic bacteria from koozh.

Ilango Shankar S Pandey Ruby R Antony Usha U

Journal of food science and technology 20160115 2

Koozh is a traditional fermented millet beverage unique to south India. Analysis of six market samples of koozh for their microbial profile resulted in 69 isolates of presumptive lactic acid bacteria (LAB). They were grouped as Leuconostoc sp., Enterococcus sp., Streptococcus sp. and Lactobacillus sp. based on morphological characteristics and biochemical tests. Eight among them showed probiotic features: resistance to acid (2.5 pH for 6 h), resistance to 0.3 % ox bile, moderate hydrophobicity ( ...[more]

PMID: 27162377

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Project description:BACKGROUND: Adhesiveness to intestinal epithelium, beneficial immunomodulating effects and the production of pathogen-inhibitory compounds are generally considered as beneficial characteristics of probiotic organisms. We showed the potential health-promoting properties and the mechanisms of probiotic action of seven swine intestinal Lactobacillus amylovorus isolates plus the type strain (DSM 20531T) by investigating their adherence to porcine intestinal epithelial cells (IPEC-1) and mucus as well as the capacities of the strains to i) inhibit the adherence of Escherichia coli to IPEC-1 cells, ii) to produce soluble inhibitors against intestinal pathogens and iii) to induce immune signaling in dendritic cells (DCs). Moreover, the role of the L. amylovorus surface (S) -layers - symmetric, porous arrays of identical protein subunits present as the outermost layer of the cell envelope - in adherence to IPEC-1 cells was assessed using a novel approach which utilized purified cell wall fragments of the strains as carriers for the recombinantly produced S-layer proteins. RESULTS: Three of the L. amylovorus strains studied adhered to IPEC-1 cells, while four strains inhibited the adherence of E. coli, indicating additional mechanisms other than competition for binding sites being involved in the inhibition. None of the strains bound to porcine mucus. The culture supernatants of all of the strains exerted inhibitory effects on the growth of E. coli, Salmonella, Listeria and Yersinia, and a variable, strain-dependent induction was observed of both pro- and anti-inflammatory cytokines in human DCs. L. amylovorus DSM 16698 was shown to carry two S-layer-like proteins on its surface in addition to the major S-layer protein SlpA. In contrast to expectations, none of the major S-layer proteins of the IPEC-1 -adhering strains mediated bacterial adherence. CONCLUSIONS: We demonstrated adhesive and significant pathogen inhibitory efficacies among the swine intestinal L. amylovorus strains studied, pointing to their potential use as probiotic feed supplements, but no independent role could be demonstrated for the major S-layer proteins in adherence to epithelial cells. The results indicate that many intestinal bacteria may coexist with and confer benefits to the host by mechanisms not attributable to adhesion to epithelial cells or mucus.

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Functional characterization and microencapsulation of probiotic bacteria from koozh.

Publications

Functional characterization and microencapsulation of probiotic bacteria from koozh.

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