Genomic variation in two gametocyte non-producing Plasmodium falciparum clonal lines.
Ontology highlight
ABSTRACT: Background
Transmission of the malaria parasite Plasmodium falciparum from humans to the mosquito vector requires differentiation of a sub-population of asexual forms replicating within red blood cells into non-dividing male and female gametocytes. The nature of the molecular mechanism underlying this key differentiation event required for malaria transmission is not fully understood.Methods
Whole genome sequencing was used to examine the genomic diversity of the gametocyte non-producing 3D7-derived lines F12 and A4. These lines were used in the recent detection of the PF3D7_1222600 locus (encoding PfAP2-G), which acts as a genetic master switch that triggers gametocyte development.Results
The evolutionary changes from the 3D7 parental strain through its derivatives F12 (culture-passage derived cloned line) and A4 (transgenic cloned line) were identified. The genetic differences including the formation of chimeric var genes are presented.Conclusion
A genomics resource is provided for the further study of gametocytogenesis or other phenotypes using these parasite lines.
SUBMITTER: Campino S
PROVIDER: S-EPMC4839107 | biostudies-literature | 2016 Apr
REPOSITORIES: biostudies-literature
Publications
Genomic variation in two gametocyte non-producing Plasmodium falciparum clonal lines.
Campino Susana S Benavente Ernest Diez ED Assefa Samuel S Thompson Eloise E Drought Laura G LG Taylor Catherine J CJ Gorvett Zaria Z Carret Celine K CK Flueck Christian C Ivens Al C AC Kwiatkowski Dominic P DP Alano Pietro P Baker David A DA Clark Taane G TG
Malaria journal 20160421
<h4>Background</h4>Transmission of the malaria parasite Plasmodium falciparum from humans to the mosquito vector requires differentiation of a sub-population of asexual forms replicating within red blood cells into non-dividing male and female gametocytes. The nature of the molecular mechanism underlying this key differentiation event required for malaria transmission is not fully understood.<h4>Methods</h4>Whole genome sequencing was used to examine the genomic diversity of the gametocyte non-p ...[more]
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