Ontology highlight
ABSTRACT:
SUBMITTER: Kamble S
PROVIDER: S-EPMC4840152 | biostudies-literature | 2016 Jan
REPOSITORIES: biostudies-literature
Kamble Suchita S Mukherjee Prasun K PK Eapen Susan S
Physiology and molecular biology of plants : an international journal of functional plant biology 20160123 1
An endochitinase gene 'ech42' from the biocontrol fungus 'Trichoderma virens' was introduced to Brassica juncea (L). Czern and Coss via Agrobaterium tumefaciens mediated genetic transformation method. Integration and expression of the 'ech42' gene in transgenic lines were confirmed by PCR, RT-PCR and Southern hybridization. Transgenic lines (T1) showed expected 3:1 Mendelian segregation ratio when segregation analysis for inheritance of transgene 'hpt' was carried out. Fluorimetric analysis of t ...[more]