Ontology highlight
ABSTRACT:
SUBMITTER: Vina-Gonzalez J
PROVIDER: S-EPMC4841332 | biostudies-literature | 2016 Apr
REPOSITORIES: biostudies-literature
Viña-Gonzalez Javier J Gonzalez-Perez David D Alcalde Miguel M
Journal of visualized experiments : JoVE 20160401 110
Directed evolution in Saccharomyces cerevisiae offers many attractive advantages when designing enzymes for biotechnological applications, a process that involves the construction, cloning and expression of mutant libraries, coupled to high frequency homologous DNA recombination in vivo. Here, we present a protocol to create and screen mutant libraries in yeast based on the example of a fungal aryl-alcohol oxidase (AAO) to enhance its total activity. Two protein segments were subjected to focuse ...[more]