A CRISPR/Cas9 system adapted for gene editing in marine algae.
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ABSTRACT:
SUBMITTER: Nymark M
PROVIDER: S-EPMC4842962 | biostudies-literature | 2016 Apr
REPOSITORIES: biostudies-literature
Publications
A CRISPR/Cas9 system adapted for gene editing in marine algae.
Nymark Marianne M Sharma Amit Kumar AK Sparstad Torfinn T Bones Atle M AM Winge Per P
Scientific reports 20160425
Here we report that the CRISPR/Cas9 technology can be used to efficiently generate stable targeted gene mutations in microalgae, using the marine diatom Phaeodactylum tricornutum as a model species. Our vector design opens for rapid and easy adaption of the construct to the target chosen. To screen for CRISPR/Cas9 mutants we employed high resolution melting based PCR assays, mutants were confirmed by sequencing and further validated by functional analyses. ...[more]
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